Abstract: Plants in Home gardens in Benue State are used locally to treat different illnesses but the frequency of their utilization is has not been documented. The study sought to identify and document the most frequently used medicinal plant species found in home gardens across the three agro-ecological zones of Benue State. About two local government areas from each of the three (3) Ecological Zones of the State were randomly selected for the study. Interviews with home garden owners (traditional medicine practitioners) were conducted using a semi-structured questionnaire. Data were subjected to descriptive statistics like frequency and percentage to determine the frequency of use of the medicinal plants. Variation in frequency of use of medicinal plants was done using one way Anova on SPSS version 20. A total of 16 plant species were found to be the most frequently used medicinal plants in home gardens in Zone A. About 18 plant species were identified as the most frequently used medicinal plants in home gardens in Zone B and 16 most frequently used medicinal plant species were identified in home gardens in Zone C. There were no significant differences in frequencies of use of medicinal plants found in home gardens of traditional medicine practitioners in Benue State at 0.05 level of significance. Most of the traditional medicine practitioners (53.33%) interviewed was elderly people. It was concluded that lack of interest by younger generations to participate in traditional medicine, can cause indigenous knowledge of plant medicine to be lost completely in the near future.