The enzyme rhodanese (thiosulfate: cyanide sulfur transferase, E.C. 2.8.1.1) is a ubiquitous enzyme known to be responsible for the biotransformation of toxic cyanide to less toxic thiocyanate using thiosulphate as the donor substrate, the aim of this research was to compare the kinetic properties of rhodanese extracted from the liver and kidney of a ram. The enzyme was assayed by measuring the amount of thiocyanate formed per minute. Rhodanese extracted from ram liver had Km values of 12.5mM±0.04 and 50mM±0.43 for Na2S2O3 and KCN respectively, while ram kidney rhodanese had Km values of 2.27mM±0.01 and 1.79mM±0.10 for Na2S2O3 and KCN respectively.. Rhodanese extracted from both the liver and kidney of ram had optimum pH and temperature as 8.5 and 30oC respectively. Rhodanese from both organs are inhibited by lead and mercury. The lower km of liver rhodanese for thiosulphate suggests that the ram liver rhodanese is more functional in transferring sulfur atom cyanide to form thiocianate.