Abstract: Teamwork is an important aspect of the work systems in a higher education institution where sharing of ideas and resources among faculty enriches the academic experience of learners. As universities and colleges in Botswana draw their faculty staff from different countries, it is imperative to investigate the impact of culture differences on teamwork among faculty staff inhigher education institutions.This qualitative studywas conducted at Gaborone University College of Law and Professional Studies (GUC). Data was collected through email questionnaire from twenty faculty staff of different nationalities in four departments. Data analysis was aided by the use of Nvivo Pro 11 qualitative data analysis software. The study found that, factors like differences in vernacular language,differences in approach to work and respect to deadlines,differences in the level of interpersonal interactions between opposite sex members, and differences in acceptable leadership styles affect teamwork among faculty staff.The study also found that, faculty staff of GUC respecttheir cultural differences, they accommodate and trust each other although the above barriers to teamwork were prevalent. The study recommended effective implementation of cultural diversity management policies to guide the staff in their formal and informal interactions in order to improve teamwork among faculty staff in GUC.