Abstract: Field trials were conducted at Dry Land Teaching and Research Farm Usmanu Danfodiyo University Sokoto during the 2015 and 2016 raining seasons to study the Effect of Inter-row spacing on the yield attribute of sesame (Sesamum indicum L.) varieties in Northern part of Sokoto, Nigeria. The treatments consisted of four levels of inter-row spacing (40, 50, 60 and 70cm) and three sesame varieties (Ex-sudan, Gulbarga Local White and Tumkur Local Black). The treatments were arranged in a randomized complete block design (R.C.B.D.) replicated three times with factorial combinations of inter-row spacing and varieties. Results of the study indicated that inter-row spacing level of 60cm consistently recorded the highest results for all the characters studied except capsule length and harvest index where 70 and 50cm inter-row spacing respectively recorded the highest result. Similarly, the use of Gulbarga Local White and Ex-sudan varieties of sesame showed superiority among the treatments in all the yield parameters investigated. From the finding of this research, it could be concluded that Gulbarga Local White variety of sesame planted at 70cm inter-row spacing gave the highest seed yield compared to Ex-sudan and Tumkur Local Black under the same conditions.