Background: Resistance to antibacterial drugs is a trouble in the worldwide public health that must be conquered immediately by finding new antibacterial drugs.Pyrazolidine-3, 5-dione derivatives of heterocyclic compounds were used as active agents against pathogenic microorganisms. This study has been carried out to rationally design three synthesized derivatives was assayed for antimicrobial potential by using in-silico molecular docking approach. Methods: Molecular docking of Isoleucyl-transfer RNA (tRNA) synthetase (IleRS) with three Pyrazolidine-3, 5-dione synthesized derivatives was carried out by AutoDock. Result: The molecular docking result revealed that synthesized compound 5(a-c) showed encouraging docking score as compared to standard ligand Gentamicin. The docking score found to be - 8.23, - 8.63, -6.89 &-8.30 kcal mol–1 respectively.