Work stress in the workplace can cause an employee to become distracted while performing tasks. Furthermore, employees will also experience depression for a period of time, either at home or at work. However, this is not a new issue and it has a number of questions and debates about existing concepts and theories. As a result, the primary goal of this research is to identify the experiences and challenges that support staff face as a result of work stress. The findings of the study show that there employees who face experiences and challenges such as harassment from colleagues, doing work for other employees, accepting work outside the scope of work, and having to bear excessive work. This research is important for understanding the job stress issues faced by support staff in private universities.