Biodiesel is an unconventional fuel composed of mono-alkyl esters also achieved essentially from the base catalyzed transesterification reaction of oils or fats. It is utilized clean haven’t require single alteration in the diesel engine and also biodiesel does not contain in consequential amount of sulphur. Consequently, biodiesel is presently the preeminent alternate for petro diesel. Biodiesel was synthesized using one step trasesterification reaction using magnesium oxide over aluminum oxide (MgO/Al2O3) at different ratio 5%, 10% and 15%. Moreoverthe biodiesel were analyzed using 6890N network GC/MS and physicochemical analysis cared out are compared according to international standardvia American Society of Testing Material (ASTMD-6751) the results obtain were: Acid value (0.1, 0.1, 0.2mgKOH/g), Density(0.64, 0.82, 0.71g/cm3) Iodine value (0.70, 0.52, 0.70mgI2/100g), Saponification value (0.10, 0.28, 0.63mgKOH/g), and Water & Sediment level (0.002, 0.002, 0.002) for sample A, B, and C, more ever the percentage yield obtained were (51%, 50%, 50.5%) for A, B, and C.