Abstract: In this study, we examined the effects of slow and rapid freezing method on the viability of cryopreserved Vero cells. For this, Vero cells were cultured and maintained following the modified protocol of Ammerman et al., (2009). After total cell counting, two frozen stocks were made simultaneously from the cell suspensions of same concentrations using DMSO and glycerol at a concentrations of 10% and preserved at liquid nitrogen temperature (-1960C) following slow and rapid freezing protocol. After 01 year of cryopreservation both frozen stocks were used providing same nutrients and environment for the revivability of the Vero cells. The cell viability analysis was performed immediately after thawing by Trypan Blue Exclusion Test. Maximum 76% in 10% DMSO (as per result section) observed in case of slow freezing whereas, it was much less in rapid freezing irrespective to the nature of cryoprotectant. It is concluded that slow freezing method could be the best choice in cryopreservation of Vero cells.