This study examined the impact of mentoring on academic performance among undergraduate students in the Department of Human Kinetics and Health Education, University of Port Harcourt. Four research questions guided the study. Descriptive survey research design was adopted for the study. Population of the study comprised 581 undergraduate students in the Department of Human Kinetics and Health Education, University of Port Harcourt out of which 116 representing 20% of the students were proportionately selected for the study. An instrument tagged “Questionnaire on Impact of Mentoring on Academic Performance among Undergraduate Students” (QIMAPUS) with r = 0.73 was used for data collection. Completed and retrieved 116 copies of the questionnaire were analyzed using mean statistics. Results showed among others that mentoring relationships provide undergraduate students the opportunities for fun and relief from daily stresses, serve as corrective therapy for undergraduate students’ emotional experiences, help undergraduate students eliminate negative perceptions about issues, help remove stigmas that undergraduate students may possess, and provide undergraduate students with enjoyable experiences. The study, therefore, recommended among others that lecturers and superior officers (mentors) in the universities should encourage students who are willing to share their feelings and self-perceptions to actively engage themselves in mentoring relationships. Lecturers and superior officers (mentors) in the universities must be able to identify students’ needs and provide the right support for them.