Abstract: The 2, 6-dibromo-1, 7-dimethyl-4-hydroxyphenyl-BODIPY was used as photosensitizer for photodynamic inactivation of two types of selected bacteria strains namely Staphylococcus aureus and Escherichia Coli. Ground state absorption spectra has shown a maximum band at 670 nm in DMF and transient absorption spectra showing the triplet state maximum bands around 472 nm and 503 nm with lifetime of (1.42±0.04)× 108 s-1. Electrospinning method was used to incorporate the dye in polystyrene matrix prior irradiation in dipped contaminated water.