Ethanolic leaf extracts of Ficus polita were investigated for the presence of phytochemical, physicochemical and their antimicrobial activities at various concentrations against some selected clinical microbes (Escherichia coli and Salmonella typhi) using standard methods. Qualitative phytochemical results showed that the ethanolic leaf extracts contained phytochemicals constituents such as terpenoids, glycosides and saponin were assayed except alkaloid, flavonoids, phenols and tannins were all absent in the extracts of Ficus polita. The physicochemical parameters of the extract revealed the pH to be 6.44 while the iodine value was 0.253 and the electric conductivity was 112.7. The results of the antibacterial activities of the ethanolic extract of Ficus polita leaves were effective on the tests organisms (Escherichia coli and Salmonella spp) at various concentrations and also sensitive against cefraxone at same concentration. In this study, Ficus polita leaves extracts exhibited varying levels of antibacterial activity against Escherichia coli and Salmonella spp. Research should be carried out using bioassay guided fractionation to identify, isolate and characterize the bioactive components of the plant extracts.