Abstract: ASI (Breast Milk) is the best food for babies that has many benefits and is designed to meet the needs of the baby according to his condition. One special component that has benefits for the growth and development of infants is alpha-lactalbumin. This study aims to determine the relationship of birth weight of babies with levels of alpha-lactalbumin in breast milk. The total sample of 46 breastfeeding mothers in the working area of the Sudiang Raya Health Center in Makassar City. Data collected; subject characteristics, infant birth weight, and mother's breast milk sample. Statistical analysis using the chi-square test. The results showed that most (97.8%) birth weight of infants was normal (≥ 2.5 kg). For the alpha-lactalbumin level, most of the subjects had alpha-lactalbumin levels with fewer content categories, namely 44 samples (95.7%), whereas subjects with normal levels only consisted of 2 samples (4.3%). From the results of the chi-square test showed that the p-value was not significant (p> 0.05), which is p = 0.829, which means there was no relationship between the birth weight of the baby with the levels of alpha-lactalbumin in breast milk. Exclusive breastfeeding requires the support of all parties, bearing in mind the technical constraints of mothers regarding the lack of in-depth socialization and education related to the benefits of various components of ASI.