Abstract: Breast cancer is the leading cause of death among women worldwide. The most effective means of prevention is by early detection via Breast self examination (BSE). This study identified the relationship between age and educational level on practice of BSE among women attending ANC in Zamfara state, Nigeria. A quantitative cross sectional design was employed using structured questionnaire. A sample of 292 participants was selected for the study using quota sampling technique. Statistical Package for Social Sciences (SPSS version 22.0, IBM Corp, New York) was used for data analysis. Chi square was used to establish significant relationships. Findings of this study suggest that there was a significant relationship (CV=5991, X2=15.19) between age of the respondents and the practice of breast self-examination. However, relationship was not established between educational level of the respondents and practice of breast self-examination (CV=7.81, X2=4.87). Therefore, there is a need for health education and awareness programme by health care practitioners regarding BSE..