The field experiment was applied in College of Agriculture/ Wasit University, Wasit, Iraq. It is located at longitude = 33.5 = 50 = 45° east and latitude = 49.8 = 29 = 32° north for the season 2022. The aim of study was to evaluate and estimate the genetic factors of the oil content of the genotypes of sesame, as well as determining the best genotype in terms of growth characteristics, yield and oil percentage within the conditions of Wasit province. Sesame seeds (Sesamum indicum L) were planting in field on 6/15/2022 and harvested on 11/25/2022. The study was conducted on oil yield, percentage and components. The results has been found that the local cultivars were significantly superior in terms of total yield as they reached highest rates, which amounted (1195.33 kg/ ha, 170.40 kg/ ha). The Iranian genotypes has been recorded the lowest rates, and the lowest rates were for the characteristic of the total yield of the genotype (1), which gave an average (459.88 kg/ ha).