Vitamin D plays a crucial role in calcium homeostasis and bone metabolism, both of which are essential for efficient orthodontic tooth movement (OTM). This study aims to explore the effects of Vitamin D deficiency on the rate and biological mechanisms of OTM. Evidence from animal models and clinical observations suggests that insufficient Vitamin D levels can impair bone remodeling by reducing osteoblastic activity and altering osteoclastic function. As a result, tooth movement may become slower and less predictable in Vitamin D-deficient individuals. Additionally, such deficiency may increase the risk of root resorption and prolonged treatment duration. Understanding the influence of Vitamin D status on OTM could aid in optimizing orthodontic treatment plans and outcomes. Further clinical studies are needed to establish clear guidelines regarding Vitamin D assessment and supplementation in orthodontic patients.