Background: Thalassemia is one of the genetic diseases in Iraq, where thousands of Iraqis suffer from this chronic disease. Tumor necrosis factor alpha (TNFα) is once of a powerful inflammatories cytokine that increases cytokine and has vital influence on immune responses. Objective: The study was aimed to investigate the relationship serum level of (TNFα- 308) correlated with pancreatic and liver disease. disorders in a subset of beta thalassemia major (BTM) people in general. Methods: Patients with pancreatic issues with thalassemia major received forty blood samples obtained, forty patients who suffering from thalassemia together with liver disorder, and Thalassemia was discovered in 40 people individuals were not diagnosed with liver or pancreatic issues, and forty samples as control were taken from people are the same age and gender. For the purpose of detecting and genotyping of TNFα-308 in all samples, the method was used T-ARMS-PCR. TNF-α serum level was detection with use ELISA. Results: The findings of estimation TNF α serum level showed that group B had higher level (137.894 ± 4.216 pg/ml) and group D had the lowest level (89.870 ±3.644 pg/ml). In addition, the findings of effect TNFα-308 genotypes on TNF α serum level of β- thalassemia major patients recorded that highest TNF α serum level in GA genotype which was (136.560±2.694 pg/ml) as compared to the GG genotype which was (106.746±2.235 pg/ml). Also the result show correlation between TNFα- 308 genotypes with TNF α serum level in all disease groups. Conclusion: Through the results we obtained, we concluded that there is relation between TNFα- 308 genotypes with TNF α serum level in A, B and C groups as well as show high TNF α serum level (pg/ml) in BTM patients as compared with control group.