Abstract: Last mile transportation is the movement of people and goods from a transportation hub to a final destination in the home or final location. In an effort to enhance last mile transportation of both goods and commuters has resulted in several investment in public transportation business, which has however become another source of problem in towns and cities. This research is however aimed at analysing the Effect of Keke Napep (Tricycle) on last mile transportation of commuters in Ojo local Government area of Lagos, in order to determine the tricycle acceptance by commuters in the area, which will also enable the researcher analyse the significance on commuters last mile transportation and the danger it pose to the commuters, which will enable the researcher make recommendations. Three hypotheses were postulated for the research, chi-square statistical tool was adopted for the first and second hypotheses which enable the researcher test the impact of tricycle on commuters last mile transportation in the area and commuters acceptance of tricycle in the study area while regression was used for the third hypothesis in order to test the relationship between commuting distance and cost. The research thus recommended the tricycle operators should avoid overloading, innovation such as the introduction of seat belt and door to prevent passengers fall off in the event of accident, sensitization of the motorcycle operators, provision of parking space and the tricycle operators should maintain a stable charging system and curtail multiple levies.